Day Two, Thursday, June 30, 2022

Advances in Small Molecule Analysis:

A Virtual Symposium


Day Two, Thursday, June 30, 2022

Advances in Small Molecule Analysis:

A Virtual Symposium


Day Two
MORNING SESSION

1 pm BST | 2 pm CEST | 8 am EDT

Introduction to ChromSoc and the Conference programme

Tony Edge, President of The Chromatographic Society (Chrom Soc), Avantor

Tony Edge is an R&D Manager heading a team of specialist scientist in developing next generation stationary phases for HPLC. He has worked in both manufacturing and also industry, having periods of employment at LGC and also AstraZeneca as well as ThermoFisherScientific, Agilent Technologies and latterly Avantor. In 2008, he was fortunate enough to be awarded the Desty memorial lecture for his contributions to innovating separation science, and in the same year also won a clinical excellence award from AstraZeneca. He has been President of the UK Chromatographic Society for 5 years.

1:15 pm BST | 2:15 pm CEST | 8:15 am EDT | KEYNOTE TALK

Designing an Exogenous Volatile Organic Compound for Biomedical Analysis

John R. Dean, Professor of Analytical and Environmental Sciences, Department of Applied Sciences, Northumbria University, Newcastle upon Tyne, UK

Bacteria naturally exude volatile organic compounds that can be analysed by a multitude of different approaches based on analytical techniques ranging from colorimetry to chromatography. However, their links to infection can be hampered by both their non-specific evolution, dependent upon growth conditions and media, or lack of specificity in the analysis. Our research in microbial detection has led to the designing, and sometimes synthesis, of exogenous volatile organic compounds that target specific enzymes characteristic of pathogenic bacteria. The exogenous volatile organic compounds have been determined using gas chromatography coupled with either a mass spectrometer or ion mobility spectrometer. This illustrated presentation will look at specific examples of important pathogenic bacteria such as Clostridium difficile, salmonella spp., listeria spp. and pseudomonas aeruginosa.

1:45 pm BST | 2:45 pm CEST | 8:45 am EDT

Turning your LC–MS “Up to Eleven”: Progress in Ultra-Rapid Bioanalysis by LC–MS

Lewis Couchman, Facility and Research Director, Analytical Services International Ltd., St. George's, University of London, London, UK

Producing results rapidly in bioanalysis is useful for many reasons. For instance, reducing turnaround times can have direct clinical benefit for results on which treatment decisions are made. In addition, maximizing instrument time can have significant operational benefits across laboratories in many sectors. LC–MS methods can be achieved in as little as 30 seconds, but there are a number of analytical parameters that must be considered to permit such rapid analysis; whilst it may be true for some applications, one cannot simply “turn their instrument to eleven” without considering the impact on the data output. This work will review the applications of ultra-rapid LC separations to-date and discuss the factors that must be considered for speeding up future methods.

2:05 pm BST | 3:05 pm CEST | 9:05 am EDT

Where Next in Separation Science? A Very Individual Look into the Future of Separation Science.

Tony Edge, Site Director (Production & R&D) Hichrom, VWR, part of Avantor, Reading, UK

Separation science has had many promises of transformational new products but not all of these have had market acceptance. This presentation will start with a brief history of chromatography before moving to review some of the bigger challenges that face separation science. From here the presentation will look at technologies that in the presenter’s eye did not quite become mainstream. From this review the presentation will then move into the future and look at the innovative technology that the chromatography community will be presented with over the next 10 years, and how if successfully implemented this could change separation science. A variety of different technology areas will be reviewed based on the current challenges that society faces, with a focus on technology that potentially could bridge the gaps between the industrial need and those that face the mass populace. Technologies that will be reviewed will include, SFC, 3D printing, designer stationary phases as well as hot water chromatography.

QUESTION AND ANSWER SESSION

2:25 pm BST | 3:25 pm CEST | 9:25 am EDT

Live panel discussion with questions from the audience moderated by Tony Edge, President of ChromSoc

AFTERNOON SESSION (SPONSOR TALKS)

3:15pm BST | 4:15pm CEST | 10:15am EDT

Octet® BLI and SPR in Biologics & Small Molecules Drug Development

David Apiyo, Manager of BioA Applications and Stuart Knowling, Senior Scientist, BioA Applications, Sartorius

Sartorius’s Octet® instruments are versatile label free platforms that can be used for critical quality attributes and other biomolecular interactions parameters evaluation through-out the drug development workflow. The Dip and Read Octet® BLI platform comes with compliance tools that include 21CFR Part 11 software, IQOQ and PQ kits that allow users in pharma to confidently develop analytical methods suitable for use in biologics quality control (QC) during therapeutic drugs development. In addition, Octet® BLI platform comes with a wide range of biosensor chemistries that enables the instrument’s use in applications ranging from small molecules to antibodies and viral particles. The Octet® SF3 on the other hand is a next generation SPR platform equipped with industry revolutionizing OneStep® Injections, unique to only to the Octet® SF3 and that utilizes a continuous analyte titration method to dramatically improve SPR-based screening for both biologics and small molecules. It provides higher content information than traditional multi-cycle SPR binding analysis and enables confident and rapid characterization of hits. In this talk, we showcase a snap-shot into case studies of the use of Octet® BLI in biologics QC and introduce the novel OneStep® gradient injection and NeXtStep® SPR approaches for small molecules screening.

3:35 pm BST | 4:35 pm CEST | 10:35 am EDT

Simultaneous LC/MS analysis of ultra-short through long chain PFAS compounds (C1-C10) using multimodal chromatography

Ron Benson, Director, Showa Denko America

Perfluoroalkyl and polyfluoroalkyl compounds (PFAS) are a structurally diverse class of manmade chemicals utilized in a variety of manufacturing processes since the 1960s. Due to their links with adverse health effects, recalcitrance, and widespread occurrence in the environment, attention is focused on the ability to detect PFAS at low concentrations. Reversed phase methods generally target long-chain PFAS (for example, PFOS) and do not simultaneously retain polar 1-3 chain carbons species. Utilizing the Shodex VT-50 2D, a sensitive and repeatable assay of PFAS including ultra-short chain (C1-C4) though long chain (C8-C10) compounds was developed to explore the retention mechanism and MS sensitivity. The chromatographic separation utilized a polyvinyl alcohol solid support with quaternary ammonium surface functional groups, capable of multi-modal retention capabilities in buffered acetonitrile eluents.

Morning Speakers

Tony Edge

President of The Chromatographic Society (Chrom Soc), Avantor

Tony Edge is an R&D Manager heading a team of specialist scientist in developing next generation stationary phases for HPLC. He has worked in both manufacturing and also industry, having periods of employment at LGC and also AstraZeneca as well as ThermoFisherScientific, Agilent Technologies and latterly Avantor. In 2008, he was fortunate enough to be awarded the Desty memorial lecture for his contributions to innovating separation science, and in the same year also won a clinical excellence award from AstraZeneca. He has been President of the UK Chromatographic Society for 5 years.

Dr. Helen Poole

Committee Member, The Chromatography Society

Dr. Poole is a committee member of the Chromatography Society and works with the team to organize events such as the recent Spring Symposium. With a PhD in biochemistry, Dr Helen Poole has over 2 decades of experience in the chromatography industry, working closely with key suppliers and supporting customers across a range of industries both nationally and internationally. Dr Poole has a particular interest in bio-pharmaceutical chromatography. In her current role at Avantor, she works closely with both suppliers and customers providing technical and application help and advice as well as bespoke training.

John R. Dean

Professor of Analytical and Environmental Sciences, Department of Applied Sciences, Northumbria University, Newcastle upon Tyne, UK

John R. Dean is currently Professor of Analytical and Environmental Sciences in the Department of Applied Sciences. He has previously held the positions of lecturer (1988), senior lecturer (1990), reader (1994), principal lecturer (1998), and associate dean for research (2004) at Northumbria University. He was awarded a personal chair by Northumbria University in August 2004. In 1998, he was awarded a D.Sc. (London) in analytical and environmental science and was the recipient of the 23rd SAC Silver Medal in 1995 (awarded by the Royal Society of Chemistry Analytical Division). John is also a Chartered Scientist, Chartered Chemist, and Fellow of the Royal Society of Chemistry. In 2015, he became Principal Fellow of the Higher Education Academy. John obtained his BSc (Hons) in chemistry from the University of Manchester Institute of Science and Technology (now Manchester University), and an MSc in analytical chemistry and instrumentation from Loughborough University. This was followed by a PhD, DIC at Imperial College, London under the supervision of Professor R.D. Snook. Postdoctoral research followed at the then Food Science Laboratory of the Ministry of Agriculture, Fisheries and Food in conjunction with Professor L.C. Ebdon at the University of Plymouth. John has been principal or co-investigator of externally funded research grants and has published extensively (over 225 refereed research papers, book chapters, and books) in analytical and environmental John’s research interests are diverse and varied. He is currently actively engaged in the development of new chromatographic methods, including ion mobility spectrometry, to assess malodour from textiles. In addition, his research has led to the development of novel approaches, based on the use of enzyme substrates, as a diagnostic tool for detection of pathogenic bacteria in food and biological matrices.

Lewis Couchman

Facility and Research Director, Analytical Services International Ltd., St. George's, University of London, London, UK

Lewis is Facility Director at Analytical Services International, based at St Georges, University of London. His current role involves overseeing the development, implementation, and routine analysis of biological samples for TDM, forensic, and clinical toxicology, and for the support of clinical trials. Lewis is the chair of the London Toxicology Group, and a committee member of the Royal Society of Chemistry Separation Science Group and the London Biological Mass Spectrometry Discussion Group.

Afternoon Speakers

David O. Apiyo, PhD

Manager of BioA Applications, Sartorius

David is Manager of BioAnalytics Applications at Sartorius. In this role he is responsible for the strategic direction of label free applications on the BLI and SPR platforms. David started out at as Field Applications Scientist in 2011 and transitioned to the marketing applications team in 2015. Prior to this David worked as a Senior Development Scientist, R&D department, at Beckman Coulter where he was involved in the development and validation of protein and antibody conjugate chemistries. David obtained his PhD in Protein Chemistry from Tulane University, New Orleans, and held several postdoctoral research positions with specialization in structural biology.

Stuart Knowling, PhD

Senior Scientist, BioA Applications, Sartorius

Dr Stuart Knowling is a Senior Applications Scientist at Sartorius and has over a decade of experience in surface plasmon resonance (SPR) and close to twenty years’ worth of experience in biophysical protein and nucleic acid production and characterization. He has previously headed multiple projects for drug companies in preparation for submissions. After research appointments at both University College San Diego and the Scripps Research Institute, La Jolla, Stuart adapted his knowledge of SPR and biophysical techniques to meet the growing demands of the Biosimilars market. Stuart is the lead author on multiple SPR guidelines.

Ron Benson

Director, Showa Denko America

Ron Benson has been the general manager of Shodex brand LC columns and detectors since 2013. His educational background includes undergraduate research in separations science with an emphasis on MALDI-TOF and graduate research in structural inorganic chemistry with 10 years of experience in X-ray diffractometry. Professional achievements include HPLC and GC-MS pharmaceutical method development and single crystal diffractometry applications.

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